Experimental data for the article "Predictable Properties of Fitness Landscapes Induced by Adaptational Tradeoffs"

We used strains from Marcusson et al. (2009) (courtesy of Douglas Huseby and Diarmaid Hughes). The strains are isogenic derivatives of MG1655, a K12 strain of the bacterium E. coli, with specific point mutations or gene deletions in five different loci: gyrA:S83L, gyrA:D87N, parC:S80I, ΔmarR, and ΔacrR. There are 32 possible combinations of these alleles, but we only used the wild type, single mutants (5 strains) and double mutants (8 strains of 10 possible combinations): LM179 (00000), LM378 (10000), LM534 (01000), LM792 (00100), LM202 (00010), LM351 (00001), LM625 (11000), LM862 (10100), LM421 (10010), LM647 (10001), LM1124 (01100), LM538 (01010), LM592 (01001), LM367 (00011). A binary sequence after the strain’s name represents the presence/absence of a particular mutated allele (order as in the above list of genetic alterations). Reference: Marcusson, Linda L., Niels Frimodt-Møller, and Diarmaid Hughes. "Interplay in the selection of fluoroquinolone resistance and bacterial fitness." PLoS pathogens 5.8 (2009).

本研究使用的菌株源自Marcusson等人（2009年）的研究（承蒙Douglas Huseby与Diarmaid Hughes惠赠）。这些菌株均为大肠杆菌（E. coli）K12菌株MG1655的同基因衍生株，在5个不同基因位点携带特异性点突变或基因缺失：gyrA:S83L、gyrA:D87N、parC:S80I、ΔmarR及ΔacrR。上述等位基因共可产生32种组合，但本研究仅使用了野生型菌株、单突变株（共5株）以及双突变株（10种可能组合中的8株），具体菌株如下：LM179（00000）、LM378（10000）、LM534（01000）、LM792（00100）、LM202（00010）、LM351（00001）、LM625（11000）、LM862（10100）、LM421（10010）、LM647（10001）、LM1124（01100）、LM538（01010）、LM592（01001）、LM367（00011）。菌株名称后的二进制序列（binary sequence）代表对应突变等位基因的存在与否，其顺序与上述遗传改变列表一致。参考文献：Marcusson LL, Frimodt-Møller N, Hughes D. 氟喹诺酮耐药性筛选与细菌适合度间的相互作用[J]. PLOS Pathogens, 2009, 5(8).