Chromatographic Profiles of Ethyl Acetate Extracts Produced by Bacillus sp. Collected from the Mangroves in the Brazilian Northeast

The genus Bacillus is Gram-positive, anaerobic or aerobic facultative bacteria, often described to be associated with the mangrove rhizosphere. Secondary products of their metabolism, coming from places affected by pollution and environmental stresses, such as mangroves, have been attracting interest from the biotechnological point of view. In view of this scenario, this work analyzed the ethyl acetate extracts of the cultured Bacillus species, previously isolated from mangroves located in the state of Sergipe, Brazil. Chromatographic profiles of seven bacterial isolates collected from two different mangroves were analyzed using gas chromatography/mass spectrometry (GC-MS) and many important compounds of biotechnological interests, mainly those derived from azo-aromatic compounds such as pyrazines, pyrrolo-pyrazines, and piperazinediones, were detected. This study is one of the first, in our knowledge, prospecting volatile or semi volatile organic compounds from ethyl acetate extracts produced by bacteria isolated from the mangroves, and intents to increase the interest in this coastal ecosystem as a source of compounds with biotechnological applications.

芽孢杆菌属（Bacillus）是一类革兰氏阳性、兼性厌氧或需氧的细菌，常被报道与红树林根际环境相关联。从受污染及环境胁迫的生境（如红树林）中分离得到的该属细菌的代谢次生产物，已在生物技术领域受到广泛关注。鉴于此研究背景，本研究对此前从巴西塞尔希培州红树林中分离得到的培养态芽孢杆菌属菌株的乙酸乙酯提取物展开了分析。研究采用气相色谱-质谱联用（gas chromatography/mass spectrometry, GC-MS）对从两处不同红树林中采集的7株细菌分离物的色谱图谱进行检测分析，成功检出多种具有重要生物技术价值的化合物，其中主要包括偶氮芳香族化合物衍生物，如吡嗪、吡咯并吡嗪及哌嗪二酮类物质。据我们所知，本研究是首批从红树林来源细菌的乙酸乙酯提取物中探查挥发性或半挥发性有机化合物的研究之一，旨在提升学界对这一海岸生态系统作为生物技术应用化合物潜在来源的关注程度。