De novo assembled transcriptome of Red Rubin (Ocimum basilicum) cultivar

RNA-Seq libraries were prepared and sequenced on an Illumina NovaSeq platform (Genewiz, Leipzig, Germany), generating 150 bp paired-end reads. Raw sequencing reads are available in the National Center for Biotechnology Information (NCBI) database (PRJNA1367508). In addition, we included in the dataset RNA-Seq libraries from RR leaves available in the Sequence Read Archive (SRA) database (accession number SRA313233). Raw reads were quality-checked with FastQC (v0.12.0) and trimmed using Trimmomatic (v0.39) to remove adapters and low-quality sequences (Q < 20; read length < 36 bp). Only high-quality reads were retained for downstream analyses. De novo transcriptome assembly was performed using the Trinity software suite (v2.13.2). Isoform reduction and redundancy removal was performed with CD-HIT.