Transcriptome analysis of rare Carpinus putoensis plants under NO2 stress

we evaluated the transcriptome using high-throughput Illumina HiSeq sequencing and related it to morphology, leaf anatomy, and physiological parameters of Carpinus putoensis putoensis under NO2 stress. The molecular mechanism of the C. putoensis NO2 stress response was evaluated using sequencing data. NO2 stress adversely affected the morphology, leaf anatomy, and total peroxidase (POD) activity. According to RNA-seq analysis, we used NCBI to compare the transcript with nine databases and get its functional annotations. We amanated amount to 2,255 million clean Illumina paired-end RNA-seq reads, and 250,200 unigene sequences was assembled by the tran-scriptome.More than 89% of C. putoensis transcripts were functionally annotated. Under NO2 stress, 1,119 genes were upregulated and 1,240 were downregulated. According to KEGG pathway and GO analyses, photosynthesis, chloroplasts, plastids, and the stimulus response are related to NO2 stress. Additionally, NO2 stress changed expression of POD families, and HPL2, HPL1, and POD genes exhibited high expression. Transcriptome analysis of C. putoensis leaves under NO2 stress supplies a reference for studying the molecular mechanism of C. putoensis resistance and elucidates NO2 stress. The obtained transcriptome data represent a treasured gene properties for future plant studies that will devote to genome annotation in future genome projects.