Water Quality Data (Extensive) from the Taylor Slough, Everglades National Park (FCE LTER), South Florida from July 1999 to Present

Water quality samples are being collected using ISCO autosamplers at all wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a Whatman GF/F filter immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP, TN, and TOC. We use these monthly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an Antec TN analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with an YSI conductivity meter. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when the threshold of = 2.5 cm of rain per hour is passed, the autosampler at that site collects a 500mL sample 30 minutes after the threshold has been reached. Rain event samples are collected, treated, and analyzed as all other water quality samples.

本研究在所有湿地样点（即除TS/Ph-9、10、11外的全部样点）采用ISCO自动采样器采集水质样品。该自动采样器配备24个1L容量的采样瓶。通过编程设置，自动采样器每3天采集一次混合水样：每18小时采集一份250mL子样品，每3天的混合样共包含4份子样品，该采样方案可覆盖黎明、正午、黄昏与午夜四个时段的样品采集。
采样工作每3至4周开展一次，采集的样品将分析总磷（TP）、总氮（TN）与盐度。在赴样点采集上述样品的同时，我们还会采集瞬时水样（grab sample），该水样在采集后立即置于冰上保存。返回实验室后，立即将部分瞬时水样通过Whatman GF/F滤膜过滤，过滤后的样品用于分析亚硝酸盐（NO₂⁻）、硝酸盐（NO₃⁻）、氨氮（NH₄⁺）、可溶性活性磷（SRP）与溶解性有机碳（DOC）等无机营养盐。未过滤的瞬时水样子样则用于分析TP、TN与总有机碳（TOC）。
我们利用这些月度采集的瞬时水样，建立TP与SRP、TN与NO₂⁻+NO₃⁻+NH₄⁺之间的相关关系。溶解性营养盐采用标准快速流动分析仪（rapid flow analyzer, RFA）技术进行测定。TP采用改良的Solorzano与Sharp（1980）方法分析；TN使用Antec总氮分析仪测定；TOC与DOC通过岛津（Shimadzu）总有机碳分析仪定量；盐度则采用YSI电导仪测量。
除常规水质监测外，本研究在所有淡水样点部署雨量触发装置，当降雨事件的时长和/或强度超过设定阈值时，即启动水样采集。按照当前的编程设置，当每小时降雨量达到或超过2.5厘米的阈值时，对应样点的自动采样器将在阈值达标后30分钟采集500mL水样。降雨事件触发的水样的处理与分析流程与其他常规水质样品一致。