A model system for assessing the ability of exon microarray to detect genes specific for malignant B-cells

The purpose of this study was to develop a quantification method that can be used to assess the ability of exon microarray to detect malignant B-cell transcripts. The data support that tumour cell concentration is an important variable with fundamental impact on gene expression pattern. We analysed eight serial dilutions of the malignant B-cell line, OCI-Ly8, into the embryonic kidney cell line, HEK293, by Affymetrix GeneChip Human Exon 1.0 ST Platform. Array data was processed by Affymetrix Exon Microarry Computional Tool. No technical replicates were performed.