Protection from infection in cyt c- and PBS- treated mice.

Protection of mice after cyt c or PBS treatment from live SPZ challenge of two independent experiments (5 mice per group in each experiment). Mice were either pre-treated iv for 3 days with 5 mg/mouse of horse cyt c per day in 100 µl of PBS or with 100 µl of PBS alone. On the last day of treatment, mice were immunized iv with 1×105PbiSPZ in 100 µl of RPMI. One week later, mice were challenged with 5×103 live PbSPZ. Parasitemia was checked at 1 and 2 weeks after challenge. Mice were considered protected if they remained parasite negative 2 weeks after challenge.