Supplementary Table 1: Urine-based regenerative RNA biomarkers for urinary bladder wound healing

<b>Supplement Table 1.</b>
The expression of genes in morning and evening<br>

<b>Abstract</b>

<b>Background:</b> This study aimed to investigate the expression of regeneration-related
genes in canine urine during bladder repair. <b>Materials &amp; methods:</b>
Canine urine samples were collected after partial cystectomy. Regenerative mRNA
of hypoxia-inducible factor (HIF), vascular endothelial growth factor (VEGF),
key stem cell transcription factors, and cholinergic signals were detected. <b>Results:</b>
HIF-1α, VEGF, CD44, interleukin-6, and prominin 1 expression in canine urine
after partial cystectomy exhibited two similar peaks at ~2 weeks. HIF-1α and
VEGF expression was higher in the afternoon than in the morning. The expression
of key stem cell transcription factors and cholinergic signals also exhibited a
rhythm along with bladder healing. <b>Conclusions:</b> The expression of
HIF-1α, VEGF, key stem cell transcription factors, and cholinergic signals
exhibited a time curve distribution during canine bladder healing. The
expression trend of some regenerative genes was similar during bladder healing,
and a cooperative effect may exist.

<b>补充表1.</b>
早晚时段的基因表达情况

<b>摘要</b>

<b>背景：</b>本研究旨在探究犬膀胱修复过程中尿液内再生相关基因的表达水平。<b>材料与方法：</b>本研究于部分膀胱切除术术后收集犬尿液样本，检测缺氧诱导因子（hypoxia-inducible factor, HIF）、血管内皮生长因子（vascular endothelial growth factor, VEGF）、关键干细胞转录因子以及胆碱能信号分子的再生mRNA表达水平。<b>结果：</b>部分膀胱切除术术后，犬尿液中HIF-1α、VEGF、CD44、白细胞介素-6以及普洛米宁1（prominin 1）的表达在约2周时出现两个相似峰值；HIF-1α与VEGF的表达在午后高于晨间；关键干细胞转录因子与胆碱能信号通路相关基因的表达亦随膀胱愈合进程呈现节律性变化。<b>结论：</b>在犬膀胱愈合过程中，HIF-1α、VEGF、关键干细胞转录因子以及胆碱能信号通路相关基因的表达呈现时间曲线分布特征；部分再生基因在膀胱愈合过程中的表达趋势相似，提示可能存在协同效应。