Data for publication Girvan et al ( 2025) Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 – 25 Å

Raw and partially processed data for Girvan, Ying and Dodson (2025) Chem Commun. Tetramethylrhodamine (TMR) is a fluorescent dye whose self-quenching has been used as a probe of multiple biological phenomena. We determine the distance-dependence of self-quenching and place bounds on the timescale of TMR dissociation. Our results validate fluorescence self-quenching as an alternative to FRET and enable future assays to be designed with confidence. Please cite our publication in any use of this data