Additional file 1: Figure S1. of Quantitative analysis of cryptic splicing associated with TDP-43 depletion

Pre-classification output of CryptEx pipeline demonstrates a variety of novel splicing events in TDP-43, FUS and hnRNP C depletion data. Every novel splicing event plotted by mean depth of reads covering the novel event against log2(fold change) between depletion and control samples. The cryptic exon classifier throws out any splicing event where the canonical intron in which the cryptic splicing event appears is represented by less than five spliced reads (purple) or where the |log2(fold change)|â

CryptEx流程（CryptEx pipeline）的预分类输出结果，在TDP-43 (Tar DNA-binding protein 43)、FUS (Fused in Sarcoma)及hnRNP C (heterogeneous nuclear ribonucleoprotein C)的敲低数据中揭示了多种新型剪接事件。以覆盖该新型剪接事件的测序读段平均深度为横轴，敲低组与对照组样本间的log₂(倍数变化)为纵轴，对每一种新型剪接事件进行绘图展示。本研究采用的隐蔽外显子分类器（cryptic exon classifier）会剔除两类剪接事件：一类是隐蔽剪接事件所在的经典内含子（canonical intron）仅由不足5条剪接读段（spliced reads）覆盖的事件（以紫色标注），另一类是其|log₂(倍数变化)|