Effect of Micro-patterned Mucin on Quinolone and Rhamnolipid Profiles of Mucoid Pseudomonas aeruginosa under Antibiotic Stress

#### Details of Pseudomonas aeruginosa biofilm dataset #### ----------------*Folder Structure*------------------------------------- This dataset contains peak intensity tables extracted from mass spectrometry imaging (MSI) data using tools, SCiLS and MSI reader. There are 2 folders in "MSI-Data-Paeruginosa-biofilms-UIUC-DP-JVS-July2022.zip", each folder contains 3 sub-folders as listed below. 1. PellicleBiofilms-and-Supernatant [Pellicle biofilms collected from air-liquid interface and spend supernatant medium after 96 h incubation period]: (1) Full-Scan-Data-96h; (2) MSMS-data-from-C7-Quinolones-96h; and (3) MSMS-data-from-C9-Quinolones-96h 2. StaticBiofilms [Static biofilms grown on mucin surface]: (1) Full-Scan-Data; (2) MSMS-data-from-C7-Quinolones; and (3) MSMS-data-from-C9-Quinolones ----------------*File name*---------------------------------------------- Sample information is included in the file names for easy identification and processing. Attributes covered in file names are explained in the example below. *Example file name "Rep1-Stat-FRD1-mPat-48-FS"* ~ Each unit of information is separated by "-" ~Unit 1 - "Rep1" - Biological replicate ( Rep1, Rep2, and Rep3) ~Unit 2 - "Stat" - Sample type (Stat = Static Biofilm, Pel = Pellicle biofilm, Sup = Supernatant) ~Unit 3 - "FRD1" - Strain (FRD1 = Mucoid strain, PAO1C = Non-mucoid strain) ~Unit 4 - "mPat" - Type of mucin surface used (mPat = patterned mucin surface, mUni = uniform mucin surface) ~Unit 5 - "48" - Sample time point (hours = 48, 72, 96) ~Unit 6 - "FS" - Scan type used in MSI (FS = high resolution full-scan, 260 = targeted MS/MS of C7 quinolones (m/z 260), 288 = targeted MS/MS of C9 quinolones (m/z 288)) ----------------*File structure*------------------------------------------ All MSI data has been exported to CSV format. Each CSV files contains information about scan number, Coordinates (x,y,z), m/z values, extraction window (absolute), and corresponding intensities in the form of a matrix. ----------------*End of Information*--------------------------------------

#### 铜绿假单胞菌生物膜数据集详情 ####
----------------*文件夹结构*-------------------------------------
本数据集包含通过SCiLS与MSI reader工具从质谱成像（mass spectrometry imaging, MSI）数据中提取的峰强度表。压缩包"MSI-Data-Paeruginosa-biofilms-UIUC-DP-JVS-July2022.zip"内包含2个主文件夹，每个主文件夹下设3个子文件夹，详情如下：
1. 浮膜生物膜与上清液（PellicleBiofilms-and-Supernatant）：指经96小时培养后，从气液界面收集的浮膜生物膜及废弃上清培养基，其子文件夹分别为：(1) 96小时全扫描数据（Full-Scan-Data-96h）；(2) 96小时C7喹诺酮类相关MSMS数据（MSMS-data-from-C7-Quinolones-96h）；(3) 96小时C9喹诺酮类相关MSMS数据（MSMS-data-from-C9-Quinolones-96h)
2. 静态生物膜（StaticBiofilms）：指在黏蛋白表面培养的静态生物膜，其子文件夹分别为：(1) 全扫描数据（Full-Scan-Data）；(2) C7喹诺酮类相关MSMS数据（MSMS-data-from-C7-Quinolones）；(3) C9喹诺酮类相关MSMS数据（MSMS-data-from-C9-Quinolones)
----------------*文件名*----------------------------------------------
样本信息已嵌入文件名中，便于识别与处理。文件名所涵盖的属性可参考下述示例进行解释：
*示例文件名"Rep1-Stat-FRD1-mPat-48-FS"*
~ 各信息单元以"-"分隔
~ 单元1 "Rep1"：生物学重复（仅包含Rep1、Rep2、Rep3三种）
~ 单元2 "Stat"：样本类型（Stat=静态生物膜，Pel=浮膜生物膜，Sup=上清液）
~ 单元3 "FRD1"：菌株（FRD1=黏液型菌株，PAO1C=非黏液型菌株）
~ 单元4 "mPat"：所用黏蛋白表面类型（mPat=图案化黏蛋白表面，mUni=均匀黏蛋白表面）
~ 单元5 "48"：样本培养时间点（单位：小时，可选值为48、72、96）
~ 单元6 "FS"：质谱成像所用扫描类型（FS=高分辨全扫描，260=C7喹诺酮类靶向MS/MS（质荷比m/z 260），288=C9喹诺酮类靶向MS/MS（质荷比m/z 288））
----------------*文件结构*------------------------------------------
所有质谱成像数据均已导出为CSV格式。每个CSV文件以矩阵形式存储以下信息：扫描编号、坐标(x,y,z)、质荷比(m/z)值、提取窗口（绝对数值）及对应强度。
----------------*信息结束*--------------------------------------