Zebrahub single cell dataset

Zebrahub first dataset is a single-cell RNA sequencing atlas a the single embryo resolution of nearly 120671 cells spanning 10 stage: from 0-somite embryos to 10 day larva (bud-, 5-, 10-, 15-, 20-, 30-somite stages, as well as 2-, 3-, 5- and 10-dpf). For each stage, we have at least 4 embryo replicates. We strive to achieve the highest possible quality; in that context, we expect the dataset to evolve to more stages and better data quality. This work is led by the Royer Lab in collaboration with CZ Biohub’s data science and sequencing platforms. It aims at providing a consistent and high-quality single-embryo resolved picture of development leveraging the latest single-cell technologies such as 10X Chromium (standard and HT) for library preparation and novaseq 6000 for sequencing. This dataset was produced in the context of upcoming preprints. In the spirit of open and accelerated science, we make this dataset available ahead of time and we require that redistribution of these data include the full text of the data release policy. <br> <strong>DATA RELEASE POLICY</strong> We aim to make sequence data rapidly and broadly available to the scientific community as a community resource. We intend to publish the work of this project in a timely fashion, and we welcome collaborative interaction on the project and analyses. However, considerable investment was made in generating these data, and we ask that you respect rights of first publication and acknowledgment as outlined in the Toronto agreement (Toronto International Data Release Workshop Authors. Prepublication data sharing. Nature. 2009 Sep 10;461(7261):168-70). By accessing these data, you agree not to publish any articles containing analyses of genes, cell types, or transcriptomic data on a whole atlas, tissue scale, or time point scale prior to the initial publication by the Chan Zuckerberg Biohub. If you wish to make use of restricted data for publication or are interested in collaborating on the analyses of these data, please email us or use the contact form. Redistribution of these data should include the full text of the data use policy.

Zebrahub首个数据集为一套实现单个胚胎分辨率的单细胞RNA测序图谱，涵盖近120671个细胞，包含10个发育阶段：从0体节胚胎至10天龄幼虫，具体包括芽期、5体节、10体节、15体节、20体节、30体节阶段，以及2、3、5、10 dpf（受精后天数）阶段。每个阶段至少设置4个胚胎生物学重复样本。

本数据集力求达到最高质量标准，我们预期该数据集后续将扩展至更多发育阶段，并进一步提升数据质量。本工作由Royer实验室主导，与陈·扎克伯格生物中心（CZ Biohub，Chan Zuckerberg Biohub）的数据科学与测序平台团队合作完成。本研究旨在借助最新单细胞技术构建一套连贯且高质量的单个胚胎分辨率发育全景图谱，所用技术包括用于文库制备的10X Chromium（标准版与高通量HT版），以及用于测序的NovaSeq 6000。

本数据集是在即将发表预印本的背景下生成的。本着开放与加速科学研究的精神，我们提前公开本数据集，并要求对本数据集进行再分发时，需完整附上本数据发布政策的全文。

<strong>数据发布政策</strong>
我们致力于将测序数据快速、广泛地向科学界开放，作为社区共享资源。我们计划及时发表本项目的研究成果，并欢迎就本项目及相关分析开展合作互动。然而，本项目在数据生成过程中投入了大量资源，我们恳请您尊重《多伦多协定》（Toronto International Data Release Workshop Authors. Prepublication data sharing. Nature. 2009 Sep 10;461(7261):168-70）中规定的首次发表权与署名要求。

若您获取本数据集，即表示您同意：在陈·扎克伯格生物中心（CZ Biohub，Chan Zuckerberg Biohub）就该完整图谱、组织尺度或时间点尺度的基因、细胞类型或转录组数据分析成果正式发表前，请勿发表包含此类分析内容的论文。若您希望使用受限数据进行发表，或有意就本数据集的分析开展合作，请通过电子邮件联系我们或使用联系表单提交请求。对本数据集进行再分发时，需完整附上本数据使用政策的全文。