Expression data from mRNA in complex with EGFR from irradiated human A549 (ATCC CCL185) cells. Homo sapiens

Immunoprecipitation of EGFR from irradiated A549 (ATCC CCL185) cells was performed in order to characterize bound mRNA species with the help of microarray analysis As control experiment, immunoprecipitation was also performed with IgG Overall design: A549 cells (ATCC CCL185) were treated with or without radiation and 24h after treatment EGFR or IgG was immunoprecipitated. To analyze gene expression changes related to irradiation in EGFR versus IgG samples, total RNA was extracted from biological triplicates (12 samples in total for the four groups) for subsequent array hybridization and microarray analysis using the Affymetrix Human Gene 2.1 ST Array chip.