five

scRNA-Seq for mouse wounded and non-wounded samples

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE140512
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Lineage negative (CD45- & CD31-) cells were isolated from uninjured skin and day 5 dorsal skin wound beds of 7-week-old AdipoqCre; mT/mG mice using FACS. This experiment describes multiple subsets of wound bed myofibroblasts and identifies that Adipoq-traced cells contribute to the myofibroblast pool in wound beds. CD45- and CD31-negative were isolated by FACS and processed for single cell RNAseq.
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2020-05-17
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