Additional file 1: of Ex vivo immunosuppressive effects of mesenchymal stem cells on Crohn’s disease mucosal T cells are largely dependent on indoleamine 2,3-dioxygenase activity and cell-cell contact

Mesenchymal stem cells with T cells from inflamed Crohn’s mucosa. Representative time-lapse imaging of a co-culture with bone marrow-derived mesenchymal stem cells of a healthy donor and T cells isolated from inflamed mucosa of a patient with Crohn’s disease. A dynamic and intense interaction is clearly evident mostly during the first 6 h, when the binding between these two cell populations is non-fixed but instead appears as a continuous attachment and detachment, after which a growing number of cells with morphological features of apoptosis (those with nuclear fragmentation and cellular swelling) and apoptotic bodies become evident. The images were serially recorded every 30 min for 12 h. Magnification: 100×. (ZIP 6780 kb)

本数据集展示了健康供体骨髓源性间充质干细胞（Mesenchymal stem cells）与克罗恩病（Crohn’s disease）患者炎症黏膜分离所得T细胞的共培养体系的代表性延时成像结果。实验观察显示，两种细胞群间的剧烈动态相互作用主要集中于实验初始的6小时内：此时二者的结合并非固定状态，而是呈现持续黏附与脱离的动态过程；在此之后，越来越多呈现细胞凋亡（apoptosis）形态学特征（即核碎裂与细胞肿胀）的细胞及凋亡小体（apoptotic bodies）逐渐显现。该序列图像以每30分钟一帧的速率连续采集12小时，放大倍数为100×。（压缩包大小：6780 KB）