CIL:9060, Nephrotoma suturalis, spermatocyte. In Cell Image Library

The events of meiosis I and II in a living insect spermatocyte beginning with the breakdown of the nuclear envelope at diakinesis through to the completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 2 min over 5 hr 18 min by a QImaging Retiga EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white).

本数据集记录了活体昆虫精母细胞中减数分裂I期与II期的完整过程，起始于终变期（diakinesis）的核被膜崩解，直至胞质分裂（cytokinesis）完成。实验以大蚊（Crane fly）物种Nephrotoma suturalis的精巢为观察材料，采用搭载液晶偏振光显微镜（liquid crystal polarized light microscopy, LC-PolScope, CRi, 马萨诸塞州沃本市）的尼康Microphot SA显微镜开展延时偏振成像，所用成像配件包括60×/1.4 PlanApo油浸物镜、1.4数值孔径油浸聚光镜及1.5倍变焦镜头。图像采集与处理流程如下：通过QImaging Retiga EXi CCD相机每2分钟采集一帧图像，总采集时长为5小时18分钟；原始图像采用5帧算法（Shribak与Oldenbourg, 2003）进行处理。影片图像以灰度值表征计算得到的相位延迟量（retardance）：相位延迟为0纳米时对应黑色，2纳米及以上则对应白色。