16S rRNA 用于 “颗粒状秸秆掺入旋耕improve soil organic carbon components，有效养分和转移细菌群落一个 浸出的肉桂土 in a East Cintakeed Cinnamon soil （华东中）”

细菌 DNA中提取从 0.5 g 新鲜土壤使用 Fast® DNA SPIN 试剂盒 （MP Biomedicals）。用细菌引物 338F （5'-ACTCCTAGGGAGGAGCA-3'） 和 806R （5'-GGACTCHVGGGTWTTAT-3'。PCR 产物使用琼脂糖凝胶 DNA 纯化试剂盒 （TaKaRa） 的高直通双端测序 DNA 样品 在 Majorbio Bio Technology Co. Ltd（中国上海）。。使用 QIIME 软件 （v 1.9.0） 对原始序列进行分析。质量过滤后，使用 QIIMEGreengene 数据库的 c 丢失参考 OTU 挑选_13_5 版本 然后，在每个级别评估每个样本的社区组成。Mothur 用于分析α多样性 细菌（Chao1 、 Simpson 和 Shannon 指数）。

Genomic DNA was extracted from 0.5 g of fresh soil using the Fast® DNA SPIN Kit (MP Biomedicals). Amplification was performed with the bacterial primers 338F (5'-ACTCCTAGGGAGGAGCA-3') and 806R (5'-GGACTCHVGGGTWTTAT-3'). PCR amplicons were purified using the Agarose Gel DNA Purification Kit (TaKaRa), and high-throughput paired-end sequencing of the DNA samples was conducted at Majorbio Bio-Technology Co., Ltd. (Shanghai, China). Raw sequencing reads were analyzed using QIIME software (v 1.9.0). Following quality filtering, OTU picking was carried out against the Greengenes database (release 13_5) using QIIME with chimeric sequences removed. The community composition of each sample was then evaluated at each taxonomic level. Alpha diversity of the bacterial community, including Chao1, Simpson and Shannon indices, was analyzed using Mothur.