Additional file 7 of LncRNA CRLM1 inhibits apoptosis and promotes metastasis through transcriptional regulation cooperated with hnRNPK in colorectal cancer

Additional file 7: Table S1. Probe sequences used to capture lncRNA-CRLM1 and LacZ in HCT116 cells. Table S2. Primer table that was used for qPCR experiment. Table S3. Primer table that was used for hnRNPK-RIP-qRT-PCR experiment. Table S4. 309 lncRNAs in MEgreen module related to metastasis using WGCNA. Table S5. DE lncRNAs between metastasis and primary tumor samples. Table S6. DEGs between CRLM1-OE and Ctrl samples. Table S7. CRLM1-bound peaks from ChIRP-seq data. Table S8. CRLM1-interacted proteins using mass spectrometry (ChIRP-MS). Table S9. HNRNPK_Ctrl-bound peaks from ChIP-seq data. Table S10. HNRNPK_CRLM1(OE)-bound peaks from ChIP-seq data. Table S11. DEGs between CRLM1-NC and Ctrl_NC samples. Table S12. DEGs between Ctrl_hnRNPK and Ctrl_NC samples. Table S13. DEGs between CRLM1_hnRNPK and CRLM1_NC samples. Table S14. CRLM1-HNRNPK up-regulated genes. Table S15. CRLM1-HNRNPK down-regulated genes.

附加文件7：表S1。用于在HCT116细胞中捕获长链非编码RNA（long non-coding RNA，lncRNA）-CRLM1与LacZ的探针序列。表S2。用于实时荧光定量聚合酶链反应（quantitative real-time polymerase chain reaction，qPCR）实验的引物列表。表S3。用于异质性核核糖核蛋白K（heterogeneous nuclear ribonucleoprotein K，hnRNPK）-RNA免疫沉淀定量实时反转录聚合酶链反应（RNA immunoprecipitation-quantitative real-time reverse transcription polymerase chain reaction，RIP-qRT-PCR）实验的引物列表。表S4。通过加权基因共表达网络分析（weighted gene co-expression network analysis，WGCNA）筛选得到的与转移相关的MEgreen模块中的309个长链非编码RNA。表S5。转移灶与原发肿瘤样本间的差异表达长链非编码RNA（differentially expressed long non-coding RNA，DE lncRNA）。表S6。CRLM1过表达（overexpression，OE）样本与对照（control，Ctrl）样本间的差异表达基因（differentially expressed genes，DEGs）。表S7。基于染色质分离纯化测序（chromatin isolation by RNA purification sequencing，ChIRP-seq）数据得到的CRLM1结合峰。表S8。通过质谱法（mass spectrometry，MS）鉴定得到的CRLM1互作蛋白。表S9。基于染色质免疫沉淀测序（chromatin immunoprecipitation sequencing，ChIP-seq）数据得到的HNRNPK对照（HNRNPK_Ctrl）结合峰。表S10。基于染色质免疫沉淀测序数据得到的HNRNPK过表达CRLM1（HNRNPK_CRLM1(OE)）结合峰。表S11。CRLM1阴性对照（negative control，NC）样本与对照阴性对照（Ctrl_NC）样本间的差异表达基因（DEGs）。表S12。Ctrl_hnRNPK样本与Ctrl_NC样本间的差异表达基因（DEGs）。表S13。CRLM1_hnRNPK样本与CRLM1_NC样本间的差异表达基因（DEGs）。表S14。CRLM1-HNRNPK上调差异表达基因（DEGs）。表S15。CRLM1-HNRNPK下调差异表达基因（DEGs）。