Transcription regulatory cascades in retinoic acid induced growth arrest of HepG2 cells. (CBX36). unidentified

To investigate ATRA-induced genes in HepG2, we analyzed gene expression at 6,12,24,36,48,60,72 hours after ATRA treatment compared with DMSO (control) treatment using illumina Sentrix Human-6 Expression BeadChip. For each time point, we hybridized four samples, two were biological replicate and another two were technical replicate. We analyzed data with three way, first and second were grouped with data of each two samples of biological replicate, and third was grouped with all data which were generated by four samples. Then, we selected genes which were included in at least, two groups, and changed expression more than 2-fold at each time point.