Study of the influence of medium composition on the motility and aggregation of the recombinant Escherichia coli strain AAEC191A/pACYCpBAD-M9 + 0.5% cassaminic acids, M9 + 0.5% cassaminic acids + 0.2% glucose, M9 + 0.5% cassaminic acids + 0.5% glucose

Microbial motility is a fundamental aspect of many microbial life cycles and is a key survival mechanism that enables microorganisms to navigate diverse and dynamic environmental conditions. This phenomenon becomes particularly important in response to changes in stimuli in time and space. The following experiment aimed to investigate how the composition of the growth medium affects the movement and formation of aggregates of recombinant Escherichia coli strains.
This study focused on several recombinant strains: BL21(DE3)/pCC90, BL21(DE3)/pACYCpBAD, BL21(DE3)/pCC90 Dra D-mut, BL21(DE3)/pCC90 D54-STOP, AAEC191A/pCC90, AAEC191A /pACYCpBAD, AAEC191A/pCC90 Dra D-mut and AAEC191A/pCC90 D54-STOP. The experimental setup involved transferring a small portion of overnight cultures of these recombinant strains to various media compositions. These media include PBS, PBS + 0.2% glucose, PBS + 0.5% glucose, LB, LB + 0.2% glucose, LB + 0.5% glucose, M9 + 0.5% cassaminic acids, M9 + 0.5% cassaminic acids + 0.2% glucose and M9 + 0.5% cassaminic acids + 0.5% glucose.
The catalog contains original video data that can be played using Olympus' CellSense software. The videos contain full layers of data characterizing the image recording process.

微生物运动性是诸多微生物生命周期的核心特征，亦是帮助微生物在多样且动态的环境条件中移动定位的关键生存机制。该现象在响应时空刺激变化时尤为重要。本实验旨在探究生长培养基的组成如何影响重组大肠杆菌（Escherichia coli）菌株的运动与聚集体形成。

本研究聚焦于多株重组菌株：BL21(DE3)/pCC90、BL21(DE3)/pACYCpBAD、BL21(DE3)/pCC90 Dra D-mut、BL21(DE3)/pCC90 D54-STOP、AAEC191A/pCC90、AAEC191A/pACYCpBAD、AAEC191A/pCC90 Dra D-mut及AAEC191A/pCC90 D54-STOP。实验流程为将这些重组菌株的过夜培养物少量接种至不同组分的培养基中。所用培养基包括磷酸盐缓冲液（PBS）、PBS + 0.2%葡萄糖、PBS + 0.5%葡萄糖、LB培养基、LB + 0.2%葡萄糖、LB + 0.5%葡萄糖、M9培养基 + 0.5%酪蛋白氨基酸、M9培养基 + 0.5%酪蛋白氨基酸 + 0.2%葡萄糖，以及M9培养基 + 0.5%酪蛋白氨基酸 + 0.5%葡萄糖。

本数据集包含原始视频数据，可通过奥林巴斯（Olympus）的CellSense软件播放。这些视频涵盖了表征图像录制全过程的完整分层数据。