lncRNA LINC01315 promotes malignancy of triple-negative breast cancer and predicts poor outcomes by modulating microRNA-876-5p/GRK5

Triple-negative breast cancer (TNBC) is a malignant tumor that threatens women’s health. Exploring novel development-associated biomarkers could help improve the survival rate of TNBC. This study evaluated the significance and mechanism of LINC01315 in TNBC progression aiming to identify a potential biomarker. There were 103 TNBC patients that provided clinical tissues in this study. The expression of LINC01315 was assessed by PCR and its association with clinical data was evaluated by statistical analyses. The <i>in vitro</i> cell experiments were conducted to estimate the biological effect of LINC01315 and its molecular mechanism. A significant upregulation of LINC01315 was observed in TNBC, which was associated with disease development and severity of patients. The upregulation of LINC01315 could be a symptom of the poor prognosis of patients. The knockdown of LINC01315 suppressed the main cellular processes of TNBC progression. Additionally, miR-876-5p was demonstrated to be a target of LINC01315 and regulate the expression of GRK5, through which LINC01315 modulated the progression of TNBC. Upregulated LINC01315 in TNBC indicated the malignant development and poor survival rate of patients. Inhibition of LINC01315 might be a potential therapeutic strategy of TNBC.

三阴性乳腺癌（Triple-negative breast cancer, TNBC）是一类威胁女性健康的恶性肿瘤。探索新型疾病进展相关生物标志物，有助于改善三阴性乳腺癌患者的生存结局。本研究聚焦LINC01315在三阴性乳腺癌进展中的作用及分子机制，以期发掘潜在诊疗生物标志物。本研究共纳入103名提供临床组织样本的三阴性乳腺癌患者，采用聚合酶链式反应（Polymerase Chain Reaction, PCR）检测LINC01315的表达水平，并通过统计学分析评估其与临床资料的相关性；同时开展体外（in vitro）细胞实验，探究LINC01315的生物学效应及分子机制。研究结果显示，三阴性乳腺癌组织中LINC01315呈显著高表达，且与患者的疾病进展及病情严重程度密切相关；LINC01315上调可提示患者预后不良。敲低LINC01315可抑制三阴性乳腺癌进展相关的核心细胞生物学过程。此外，本研究证实miR-876-5p是LINC01315的靶标分子，可调控GRK5的表达，LINC01315正是通过该轴调控三阴性乳腺癌的进展。三阴性乳腺癌组织中LINC01315高表达可提示患者恶性疾病进展与不良生存结局，抑制LINC01315或可成为三阴性乳腺癌的潜在治疗策略。