Description of an automatic copulation induction system used to establish a free-mating laboratory colony of Nyssorhynchus deaneorum from Brazil

BACKGROUND Nyssorhynchus deaneorum is a potential malaria vector because it has been shown to be competent to transmit Plasmodium vivax and Plasmodium falciparum, and because it exhibits antropophilic and endophilic behaviors in some regions of the Amazon. This profile makes Ny. deaneorum a useful mosquito for experiments that model Plasmodium-vector interactions in the Amazon. OBJECTIVE Herein we describe how a free-mating colony of Ny. deaneorum has been established using an automated light stimulation system. METHODS Mosquitoes were captured in São Francisco do Guaporé, Rondônia. The F1 generation was reared until adult emergence at which point copulation was induced using an automatic copulation induction system (ACIS). FINDINGS After four generations, natural mating and oviposition began to occur without light stimulation. The number of pupae and adult mosquitoes increased from the F5 to F10 generations. The new Ny. deaneorum colony exhibited susceptibility to P. vivax. MAIN CONCLUSIONS Automated light stimulation is an effective method for establishing an Ny. deaneorum colony under laboratory conditions as it produces enough adults to create a stenogamic colony. The establishment of a stable, P. vivax-susceptible colony of Ny. deaneorum makes it possible to model parasite-vector interactions and to test novel drug therapies that target parasite development in mosquitoes.

研究背景：迪安诺纽索按蚊（Nyssorhynchus deaneorum）是一种潜在的疟疾传播媒介，已有研究证实其可传播间日疟原虫（Plasmodium vivax）与恶性疟原虫（Plasmodium falciparum），且在亚马逊流域部分区域表现出嗜人习性与家栖行为。该特性使Ny. deaneorum成为模拟亚马逊地区疟原虫-媒介互作实验的理想蚊虫模型。
研究目的：本文详述如何通过自动化光刺激系统建立迪安诺纽索按蚊的自由交配种群。
研究方法：蚊虫捕获于巴西朗多尼亚州瓜波雷河畔圣弗朗西斯科（São Francisco do Guaporé, Rondônia）。将F1代饲养至成虫羽化阶段，随后利用自动交配诱导系统（Automatic Copulation Induction System，ACIS）诱导其交配。
研究结果：传至第4代时，无需光刺激即可自然发生交配与产卵。F5至F10代的蛹与成虫数量逐步提升。新建的迪安诺纽索按蚊种群对间日疟原虫表现出易感性。
主要结论：自动化光刺激法可在实验室条件下有效建立迪安诺纽索按蚊种群，能够获得足够数量的成虫以构建狭室交配种群。稳定建立的间日疟原虫易感型迪安诺纽索按蚊种群，为模拟疟原虫-媒介互作机制以及测试靶向蚊虫体内疟原虫发育的新型药物疗法提供了可行的研究平台。