De novo assembled contigs for a cryoconite microcosm study - Canada Glacier Cryoconite hole sample - NCBI BioProject PRJNA1315162

This dataset has short read sequences from of communities exposed to nutrient enrichment under microcosm conditions. The original communities came from cryoconite hole sediments from Canada Glacier, Taylor Valley, Antarctica. Homogenized sediments were then distributed among 20 sterile Petri dishes (60 mm), each with 25 g dry weight equivalent (31.25 g wet sediment based on 25% water content) and assigned to five experimental treatments. Treatments consisted of 1) Bulk, sediments that received neither water nor nutrients, 2) Control, sediments that received additions of 1 ml of water, 3) +N, sediments that received 1 ml of ammonium nitrate solution to achieve 4 µmol N * g soil-1, 3) +P, sediments that received 1 ml of sodium phosphate dibasic solution to achieve 2 µmol P * g soil-1, and 5) +NP, sediments that received 1 ml of combined ammonium nitrate and sodium phosphate dibasic to achieve 4 µmol N + 2µmol P * g soil-1. Each treatment was replicated five times and incubated in a freeze-thaw growth chamber with temperatures cycling between 1°C and 3°C and light between ~100 Lux (5 hrs/day) and ~10,000 Lux (19 hrs per day) to imitate natural cryoconite holes conditions during austral summers.

本数据集包含了微宇宙（microcosm）条件下经营养富集处理的群落的短读长测序序列（short read sequences）。原始群落取自南极洲泰勒谷加拿大冰川的冰尘穴（cryoconite hole）沉积物。将均质化后的沉积物分装至20个无菌60mm培养皿中，每皿分装相当于25克干重的沉积物（按25%干基含水率计算，对应湿沉积物重量为31.25克），并将其划分为5组实验处理：1. 空白组（Bulk）：不添加水分与营养物质的沉积物；2. 对照组（Control）：添加1毫升去离子水的沉积物；3. +N组：添加1毫升硝酸铵（ammonium nitrate）溶液，使沉积物氮含量达到4微摩尔每克干重；4. +P组：添加1毫升磷酸氢二钠（sodium phosphate dibasic）溶液，使沉积物磷含量达到2微摩尔每克干重；5. +NP组：添加1毫升硝酸铵与磷酸氢二钠混合溶液，使沉积物氮、磷含量分别达到4微摩尔每克干重与2微摩尔每克干重。每组处理设置5个生物学重复，随后置于冻融培养箱（freeze-thaw growth chamber）中培养：温度在1℃至3℃之间循环波动，光照强度在约100勒克斯（Lux，每日照射5小时）至约10000勒克斯（每日照射19小时）之间动态调整，以模拟南极夏季冰尘穴的自然环境条件。