Fecal Microbiota in the MOG/CFA induced EAE mouse model as compared to OVA/CFA immunized mice

Fecal samples were collected from mice that were immunized with MOG/CFA or, as a control, immunized with OVA/CFA, at the day of immunization (0 d.p.i), 8 days post immunization (8 d.p.i) and 15 days post immunization (15 d.p.i). DNA in the feces was extracted using a QIAamp Fast DNA Stool Mini Kit (Qiagen). Amplicons spanning variable region 4 (V4) of the bacterial 16S rRNA gene were generated with primers containing barcodes (515F, 806R) from the Earth Microbiome project using HotMaster Taq and HotMaster Mix (QuantaBio) and sequenced on an Illumina MiSeq platform at the Harvard Medical School Biopolymer Facility