Profile of volatile compounds released by Waitea circinata against Magnaporthe oryzae under different periods and temperatures

ABSTRACT Rice blast caused by the Magnaporthe oryzae pathogen is a major disease in this crop, and may cause devastating losses. This study aimed to investigate the profile of Waitea circinata mycorrhiza volatile compounds in antagonism to M. oryzae, under different growth periods and temperatures, using a completely randomized design. Volatile organic compounds were extracted by headspace solid phase microextraction and analyzed by gas chromatography mass spectrometry, while multidimensional scaling was used to compare the produced volatile organic compounds. The main compounds responsible by the antagonism to M. oryzae were longifolene, trans-β-farnesene, (Z)-α-bisabolene and δ-amorphene, which can be used as biofungicides and incorporated into rice blast management strategies.

摘要 由稻瘟病菌（Magnaporthe oryzae）引发的稻瘟病是水稻的主要病害之一，可造成毁灭性产量损失。本研究采用完全随机设计，探究不同生长周期与温度条件下，环状瓦氏霉（Waitea circinata）菌根挥发性化合物对稻瘟病菌的拮抗活性谱。研究通过顶空固相微萃取（headspace solid phase microextraction）提取挥发性有机化合物，采用气相色谱-质谱联用法（gas chromatography mass spectrometry）进行成分分析，并利用多维尺度分析（multidimensional scaling）对比所获得的挥发性有机化合物组分。对稻瘟病菌具有拮抗作用的核心活性成分为长叶烯（longifolene）、反式-β-法尼烯（trans-β-farnesene）、(Z)-α-红没药烯（(Z)-α-bisabolene）以及δ-紫穗槐烯（δ-amorphene），上述化合物可作为生物杀菌剂应用，并可纳入稻瘟病防控策略体系。