Vascular integrity is essential for maintaining tissue homeostasis II

Purpose: analyze the transcriptomic difference of endothelial cells and pericytes in PBS, LPS or LPS + Riociguat treated lungs Methods: Gucy1a1-EGFP mice were given PBS or LPS treatment via trachea instillation. Vehicle or Riociguat oral administation was performed at every 9 hours. 24h later, lungs were dissociated and endothelial cells and pericytes were isolated by flow cytometry, then directly processed to RNA extraction and library construction Results: Riociguat can restore LPS-induced lung injury by inhibiting pericytes activation and maintain vascular homostatsis mRNA profiles of mice lung endothelial cells and pericytes were generated by sequencing.