GFP-Rab11A intracellular movement in Nocodazole (16 µm) treated A549 cells

TIFF series of diSPIM datasets, fused and deconvolved, from A549 GFP-Rab11A cells treated with Nocodazole (16 µm). Each TIFF file is a full cell volume of 50 planes, with isotropic resolution of 330nm, each .zip file is an independent replicate with at least 1 or 2 cells analyzed for 3D tracking of cytoplasmic foci with 500 time stamps. These data correspond to Supplementary Movie 4 and analysis of these movies provided data presented in Figure 3 and Supplementary Figure 4. <br>Supplementary Movie 4: A549 GFP-Rab11A cells under nocodazole treatment. <br>Figure 3: Motion Dynamics of Rab11A under the effect of cytoskeletal depolymerizing drugs.<br>Supplementary Figure 4: Impact of skeletal depolymerizing drugs on the transport of Rab11A.

本数据集为经去卷积与融合处理的双视图平面照明显微镜（diSPIM）数据集的标签图像文件格式（TIFF）序列，样本为经诺考达唑（Nocodazole，16 µM）处理的A549 GFP-Rab11A细胞。每个TIFF文件对应包含50层的完整细胞三维体数据，各向同性分辨率为330 nm。每个.zip压缩包为独立重复实验，包含至少1~2个细胞的分析数据，用于对胞质焦点开展500个时间点的三维追踪。本数据集对应补充视频4，对该视频的分析结果即为图3与补充图4所展示的数据。
补充视频4：经诺考达唑处理的A549 GFP-Rab11A细胞
图3：细胞骨架解聚药物作用下Rab11A的运动动力学
补充图4：细胞骨架解聚药物对Rab11A转运的影响