Dataset for: Bacteriocin ASM1 is an O/S-diglycosylated, plasmid-encoded homologue of glycocin F

<i>Lactobacillus plantarum</i> A-1 secretes bacteriocin ASM1, with a calculated monoisotopic mass of 4642.8454 Da (based on the 43 amino acid sequence) and a measured mass of 5044.9727 Da. Here we show that ASM1 is a glycocin (glycosylated bacteriocin) with <i>O</i>- and <i>S</i>-linked <i>N</i>-acetylglucosamine moieties identical to glycocin F (GccF), and that the roles of specific structural features of ASM1 are very similar to GccF. Analysis of <i>Lb. plantarum</i> A-1 genomic DNA sequences showed that <i>asm</i> gene organisation (<i>asmH←→asmABCDE→F</i>) is identical to the <i>gcc</i> gene cluster, and that the <i>asm</i> genes reside on the novobiocin-curable 11,905-bp plasmid pA1_ASM1. Amino acid sequence identity averages ~69% across all Asm and Gcc proteins, and is highest for the identical ASM1/GccF peptide scaffold residues 1-30.

植物乳杆菌（*Lactobacillus plantarum*）A-1可分泌细菌素ASM1，其基于43个氨基酸序列计算得到的单同位素质量为4642.8454 Da，实测质量为5044.9727 Da。本研究证实，ASM1是一种糖细菌素（glycocin，即糖基化细菌素），其携带与糖细菌素F（GccF）完全一致的O-连接与S-连接N-乙酰葡糖胺修饰基团，且ASM1特定结构特征的功能与GccF高度相似。对植物乳杆菌（*Lb. plantarum*）A-1的基因组DNA序列分析结果显示，*asm*基因的排布（*asmH* ←→ *asmABCDE* → *asmF*）与*gcc*基因簇完全一致，且*asm*基因坐落于可被新生霉素消除的11905 bp质粒pA1_ASM1上。所有Asm与Gcc蛋白的氨基酸序列同源性平均约为69%，其中ASM1/GccF的1~30位肽骨架残基完全一致，同源性最高。