Nunu Dairy Microbiome Phage DM-1

With the advent of next-generation sequencing (NGS) on metagenomes, the elucidation of all genetic material from microbiomes has prompted a renewed interest towards uncultivated members of the virosphere. We describe the discovery of a novel phage from a metagenomic dataset on the West African fermented dairy product, nunu, with a custom bioinformatics workflow to potentially serve as a biocontrol agent against pathogenic <em>E. coli</em>. Initial dataset of ERR2014814 from NCBI was first subjected to Kraken2 to extract novel sequencing reads for further <em>de novo</em> assembly into contigs.

随着下一代测序（NGS）技术在宏基因组研究中的普及，解析微生物组全套遗传物质的工作再度唤起了学界对病毒圈未培养病毒类群的研究兴趣。本研究通过定制化生物信息学流程，从西非发酵乳制品nunu的宏基因组数据集中发现了一株新型噬菌体，该噬菌体有望作为对抗致病性大肠杆菌（E. coli）的生防制剂。研究首先对NCBI数据库中编号为ERR2014814的初始数据集应用Kraken2工具进行分类分析，以提取新型测序读段，进而开展后续的从头组装（de novo）以获得重叠群（contigs）。