Cell-type and subcellular compartment specific isolation of miRNAs directly from the mouse brain by in vivo AGO-APP

We derived two transgenic mouse lines expressing a T6B peptide either in the whole cytoplasm or targeted at the post-synapse, in order to perform AGO-APP directly from mouse tissues. We used these mice first to compare the expression of AGO-bound micro-RNAs in 2 types of neuron, the olfactory bulb inhibitory interneurons and the cortical excitatory neurons, and second to analyze the post-synaptic targeting of micro-RNAs in these 2 neuron types.