Supplementary data for: Transposon mutagenesis identifies cooperating genetic drivers during keratinocyte transformation and cutaneous squamous cell carcinoma progression

Supplementary Note 1:

S1 Text: Oncogenomic comparisons between SB candidate Trunk driver genes and their direct orthologs in human Cancer Gene Census; Pyrosequencing analysis of SB-driven keratinocyte cancer models; References.

Supplementary Figures 1-11:

S1 Fig: Overview of genetic crosses to generate SB|Trp53|Onc3 mouse model.
S2 Fig: SB insertion patterns in activated and inactivated drivers.
S3 Fig: Evaluating the reproducibility of SBCapSeq results from bulk cuSCC and normal skin specimens.
S4 Fig. Hierarchical two-dimensional clustering of recurrent events in cuKA and cuSCC.
S5 Fig. Curated biological pathways and processes enriched within SB-induced cuSCC.
S7 Fig: ZMIZ1 metagene within the TCGA Head & Neck Squamous Cell Carcinoma (hnSCC) RNA-seq dataset.
S8 Fig: Clonally selected SB insertions affect trunk driver proto-oncogene expression in SB-cuSCC genomes.
S9 Fig: Clonally selected SB insertions affect trunk driver genes by inactivating expre...

补充说明1：

S1 文本：睡美人（Sleeping Beauty, SB）候选躯干驱动基因与人类癌症基因普查（Cancer Gene Census）中其直接同源基因的肿瘤基因组学比较；睡美人（Sleeping Beauty, SB）诱导的角化细胞癌模型的焦磷酸测序分析；参考文献。

补充图1-11：

S1 图：构建SB|Trp53|Onc3小鼠模型的遗传杂交方案概览。
S2 图：激活与失活驱动因子的SB插入模式。
S3 图：评估批量皮肤鳞状细胞癌（cuSCC）标本与正常皮肤标本的SBCapSeq检测结果可重复性。
S4 图：皮肤角化棘皮瘤（cuKA）与皮肤鳞状细胞癌（cuSCC）复发事件的层级二维聚类分析。
S5 图：SB诱导的皮肤鳞状细胞癌（cuSCC）中富集的精选生物学通路与生物学过程。
S7 图：癌症基因组图谱（The Cancer Genome Atlas, TCGA）头颈部鳞状细胞癌（hnSCC）RNA测序（RNA-seq）数据集内的ZMIZ1元基因。
S8 图：经克隆筛选的SB插入变异可影响SB诱导皮肤鳞状细胞癌（SB-cuSCC）基因组中躯干驱动原癌基因的表达。
S9 图：经克隆筛选的SB插入变异通过失活表达……影响躯干驱动基因。