Acute exposure water chemistry data

To fully assess the impacts of Deepwater Horizon (DWH) oil on the vertebrate stress response and the trajectory for recovery, our research over a series of experiments integrated molecular, cellular, gland/organ and whole animal physiological and behavioral studies using a benthic marine teleost, the Gulf toadfish, a resident of the Gulf of Mexico. These studies evaluated multiple pathways (e.g., pituitary fatigue, reduced receptor sensitivity, disruption or overstimulation of cortisol biosynthesis) and the consequence of these changes at the whole animal level (e.g., changes in carbohydrate metabolism, immune capacity and responses to natural stresses such as predation and social interaction). In this series of experiments, the pathways and endpoints were evaluated during short-term exposures to DWH oil. Water parameters and water chemistry were analyzed. Each exposure was followed by a complementary recovery period (i.e., no oil or PAH exposure) to assess the recovery trajectory. These experiments were completed at Rosenstiel School of Marine and Atmospheric Science (RSMAS), University of Miami, Marine Technology & Life Sciences Seawater Complex (MTLSS) Building, room 211 between January 2018 to December 2018.

为全面评估深水地平线（Deepwater Horizon, DWH）原油对脊椎动物应激反应的影响及其恢复轨迹，本研究通过一系列实验，以栖息于墨西哥湾的底栖海硬骨鱼类——海湾豹蟾鱼为实验对象，整合了分子、细胞、腺体/器官及整体动物层面的生理学与行为学研究。本系列实验评估了多条应激通路（如垂体疲劳、受体敏感性降低、皮质醇生物合成紊乱或过度刺激），并考察了上述变化在整体动物层面引发的后果，包括碳水化合物代谢改变、免疫能力变化，以及对捕食、社交互动等自然应激源的响应变化。在本系列实验中，研究人员在受试生物短期暴露于DWH原油期间，对上述应激通路与观测终点进行了评估，并同步分析了水体参数与水化学特征。每一轮原油暴露实验结束后，均设置配套的恢复周期（即无原油或多环芳烃（Polycyclic Aromatic Hydrocarbons, PAH）暴露的阶段），以评估受试生物的恢复轨迹。本系列实验于2018年1月至12月间，在迈阿密大学罗森斯蒂尔海洋与大气科学学院（Rosenstiel School of Marine and Atmospheric Science, RSMAS）海洋技术与生命科学海水综合体（Marine Technology & Life Sciences Seawater Complex, MTLSS）211室完成。