Saccharomyces cerevisiae Transcript Isoform mapping

Application of TIF-Seq (Transcript IsoForm Sequencing) to S.cerevisiae. The method was applied to simultaneously identify the 5' capped mRNA transcription start site and the 3' polyadenylation site in different conditions将转录本异构体测序（Transcript IsoForm Sequencing，简称TIF-Seq）应用于酿酒酵母（S.cerevisiae）。该方法可在不同实验条件下同时鉴定5'端加帽mRNA的转录起始位点与3'端多聚腺苷酸化位点。