Transcriptomic analysis of Erwinia amylovora bacteriophage insensitive mutants

Phage therapy has garnered significant attention due to the rise of life-threatening multidrug-resistant pathogenic bacteria and the growing awareness of the transfer of resistance genes between pathogens. In light of this, phage therapy applications are now being extended to target plant pathogenic bacteria, like Erwinia amylovora that causes fire blight in apple and pear orchards. Understanding the mechanisms of phage resistance development is crucial for enhancing the effectiveness of phage therapy. Despite the challenges of naturally developing a bacteriophage resistant mutant (BIM) of E. amylovora (without traditional mutagenesis methods), this study successfully created a BIM mutant against the podovirus ?Ea46-1-A1. The parent strain, E. amylovora D7, and the BIM mutant, B6-2 were compared at the transcriptomic level. Overall design: RNASeq of E. amylovora wild-type and BIM strains