Real-time qPCR measurement of senescence-up-regulated genes (SURGs) and senescence-down-regulated genes (SDRGs) mRNA levels in tissue used for ChIP-seq.

Total RNA was harvested from the same leaves used in the ChIP-seq analysis, and real-time qPCR was performed using ACT2 as a reference. SURGs and SDRGs were identified from published microarray data [4]. Fold-induction at 52 d for SURGs and at 23 d for SDRGs is reported. Eight of 8 tested SURGs were up-regulated by at least 15-fold, while 6 of 7 tested SDRGs were down-regulated by at least 24-fold.