ChIP-seq analysis of H3[delta]N genomic localization in U937 cells, primary monocytes, and monocyte-derived macrophages

ChIP-seq analysis reveals widespread H3[delta]N distribution across the genome in U937 cells, and primary monocytes, which is largely absent in fully differentiated macrophages. Overall design: ChIP-seq analysis of i) U937 cells with two biological replicates; ii) peripheral blood monocytes from three healthy volunteers; and iii) the matching monocyte-derived macrophages from ii) using antibodies against bulk h3 or H3[delta]NThr22