Additional file 10: of A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling

Curve-fitting results for individual dose–response data in Fig. 3 and results of ANOVAs shown in Fig. 3 and Additional file 8: Figure S6. For RHO, LWS cone opsin, SWS cone opsin, RL3Sc, RL3Am, and RL3m6L2, the irradiance–response data from each individual trial was analyzed with an F-test comparing a three-parameter sigmoid model (test hypothesis) to a flat-line model (null hypothesis), with significance threshold p < 0.05 (same analysis as with the pooled data). a Details of curve-fitting to individual irradiance–response data, including the F-statistic, degrees of freedom, and corresponding p value for each test, and the best-fitting parameters that define the irradiance–response curve: bottom, top (response amplitude), and logEC50 (sensitivity). b Relevant details of the ANOVA tests in Fig. 3d. c Relevant details of the ANOVA tests in Fig. 3e. d Relevant details of ANOVA test in Additional file 8: Figure S6a. e Relevant details of ANOVA test in Additional file 8: Figure S6b. (XLSX 24 kb)