DNA methylation profiles of cells commonly found in bronchoalveolar lavage

Genome wide DNA methylation profiling of 4 cell populations purified from paediatic bronchoalveolar lavage samples. The cell types profiled are alveolar macrophages, granulocytes, lymphocytes and alveolar epithelial cells. Fluorescence-activated single cell sorting was used to purify the cell populations of interest using previously described methods (Shanthikumar, AJRCMB, 2020 ;63(2):152-159). Genome wide DNA methylation profiling was performed via the EPICArray. These data can be used for reference based deconvolution of cell proportion of bronchoalveolar lavage samples. DNA extracted from raw BAL (n=6) was also profiled using the EPICArray, allowing for validation of reference based deconvulution. Bisulphite converted DNA from a total of 20 samples were hybridised to the Illumina Infinium MethylationEPIC BeadChip. 14 samples are made up of alveolar macrophages (n=5), granulocytes (n=4), lymphocytes (n=3), and alveolar epithelial cells (n=2). 6 samples are raw BAL.