Acacia crassicarpa Raw sequence reads

Acacia crassicarpa is a tropical forestry species that is increasingly being planted in parts of Southeast Asia. We developed twelve nuclear microsatellite markers for A. crassicarpa using next generation sequencing, which are optimised for assay in three multiplex sets. We tested the 12 loci on 34 unique A. crassicarpa samples and found that polymorphism ranged from four to eight alleles per locus (average = 5.8). The loci were easy to score (in terms of binning) and their reproducibility and PCR success rate were both high. Given their usability and polymorphism we believe that these 12 loci will be useful for DNA profiling and mating sytem analysis with direct application in breeding programs and to the conservation of wild populations. Six of the markers also amplified products in the related A. mangium and A. auriculiformis, of which four were polymorphic in the six samples. Thus these markers also add to the genomic resources available in Acacia more broadly.