SNP data for Syringa vulgaris genotypes

Common lilac (Syringa vulgaris L.) is a popular landscaping plant. In the present study, its genotypes were investigated using genotyping-by-sequencing (GBS) methodology. Our aim was to obtain a large set of SNP markers, to reveal the precise identities of the investigated S. vulgaris accessions, and to discover genetic relationships among them. The studied plant material included local Finnish, previously unidentified accessions, known reference cultivars, and so-called historical accessions i.e., old shrubs growing in historic cultural landscapes. We intended to verify cultivar names for some valuable local common lilac accessions and to provide insights into the history of common lilac cultivation in Finland. In the analyses, we used a set of 15,007 SNP markers. First, polymorphic information contents (PIC) were calculated (mean 0.190, range 0.012–0.500 per marker). Then, to investigate genetic relationships among genotypes, a phylogenetic tree was constructed, and a principal coordi..., Genotyping by sequencing of 85 S. vulgaris samples was conducted by using a genome profiling service by Diversity Arrays Technology Pty Ltd. (Canberra, Australia, http://www.diversityarrays.com), following the DArT genotyping protocol. Pairwise genetic distances between S. vulgaris genotypes were calculated by GenAlEx 6.5. Based on the distance values, a phylogenetic tree was constructed and visualized using the Neighbor-Joining method with Mega 6. GenAlEx was also used to conduct a principal coordinate analysis (PCoA) to investigate genetic relationships among genotypes. A Bayesian analysis of population structure using software Structure 2.3.4. was carried out to determine the number and distribution of genetic clusters among samples., Excel

欧丁香（Syringa vulgaris L.）是广受喜爱的园林观赏植物。本研究通过测序分型（genotyping-by-sequencing, GBS）技术对其基因型展开分析，旨在获取大量单核苷酸多态性（single nucleotide polymorphism, SNP）标记，明确供试欧丁香种质的准确身份，并揭示其遗传亲缘关系。供试材料涵盖芬兰本土此前未被鉴定的种质、已知参考品种，以及所谓的"历史种质"——即生长于历史文化景观中的古老灌木。本研究旨在验证部分珍贵本土欧丁香种质的品种名称，并为芬兰欧丁香栽培历史提供研究视角。分析过程中共使用15007个SNP标记：首先计算多态性信息含量（polymorphic information content, PIC），平均值为0.190，单个标记的取值范围为0.012~0.500；随后为探究基因型间的遗传亲缘关系，构建系统发育树并开展主坐标分析。本研究通过澳大利亚多样性阵列技术有限公司（Diversity Arrays Technology Pty Ltd., 澳大利亚堪培拉，http://www.diversityarrays.com）提供的基因组分型服务，并遵循DArT分型流程，对85份欧丁香样本进行测序分型。利用GenAlEx 6.5软件计算欧丁香基因型间的两两遗传距离；基于该距离矩阵，通过Mega 6软件采用邻接法（Neighbor-Joining）构建并可视化系统发育树。GenAlEx 6.5同时用于开展主坐标分析（principal coordinate analysis, PCoA）以探究基因型间的遗传亲缘关系。利用Structure 2.3.4软件开展群体结构的贝叶斯分析，以确定样本中遗传簇的数量与分布情况。Excel