Immunoprecipitation mass spectrometry of SPARK-mNG

SPARK is an AGC kinase found specifically in apicomplexan parasites. We have previously shown that SPARK is essential for parasite calcium mobilization, egress, and invasion. Here we have performed immunoprecipitation followed by mass spectrometry to determine interaction partners of SPARK. We identify a hypothethical protein that interacts with SPARK, which we term SPARKEL.