Video recordings associated vith the pubblication: Focused ultrasound neuromodulation on MEA

This dataset contains the video recording associated with the publication. In the first video, the multiwell MEA has been used to test the temperature variation during the period of US-ON. A thermocouple type K was placed at the bottom of the well and the ultrasound transducer immersed in the well. The input voltage was the same as the one used during the cell experiment and the MEA plate was placed on top of a hot plate to reproduce the experimental conditions. After 60 seconds of ultrasound the temperature increase was 0.8 °C. In the second video, the heat profile generated by the ultrasound transducer used for the ultrasound neuromodulation experiment is observed using the Schlieren system. The trasnduecr is placed in the same Schlieren setup configuration used to validate the ultrasound field and a thermocouple is placed close to the tip of the transducer. The ultrasound is turned on for 60 seconds with a driving voltage 4.5 times higher than the one used during the cell experiment. It can be seen that the heat profile generated by the trasducer remains rather shallow the tip of the transducer and the water surface, suggesting that the heat does not reach the neuronal culture and it is dispersed at the liquid surface, rather than towards the neurons.

本数据集包含与该学术论文相关的视频记录。在第一段视频中，研究人员采用多孔微电极阵列（multiwell MEA）测试了超声开启（US-ON）阶段的温度变化：将K型热电偶（thermocouple type K）置于孔槽底部，并将超声换能器（ultrasound transducer）浸入孔槽内。输入电压与细胞实验中所用参数保持一致，同时将微电极阵列板放置在加热台（hot plate）之上以还原真实实验条件。超声开启60秒后，温度升高了0.8 ℃。在第二段视频中，研究人员通过纹影系统（Schlieren system）观测了用于超声神经调控（ultrasound neuromodulation）实验的超声换能器所产生的热分布。换能器置于与超声场验证实验完全相同的纹影装置配置中，并将热电偶置于换能器探头附近。本次超声开启时长为60秒，驱动电压为细胞实验所用电压的4.5倍。观测结果显示，换能器产生的热分布在换能器探头与水面之间相对较浅，表明热量并未抵达神经元培养区域，而是在液面处消散，而非朝向神经元方向传导。