Supplemental Material for Goetsch and Strome, 2022

Supplemental information includes supplemental Materials and Methods. Figure S1 contains full western blots presented in Figure 2D. Figure S2 contains full western blots presented in Figure 3B. Figure S3 contains additional DREAM target genes tested using qPCR for ChIP and sequential ChIP analyses to complement Figure 6. Antibodies used for western blot and ChIP analyses are available in Supplemental Table S1. Primers used for cloning and qPCR are available in Supplemental Table S2. DESeq2 differential gene expression analysis data are available in Supplemental Table S3

补充信息包含补充实验材料与方法。
图S1包含图2D所展示的完整免疫印迹（western blot）结果。
图S2包含图3B所展示的完整免疫印迹结果。
图S3包含额外的DREAM靶基因，这些靶基因经实时定量聚合酶链式反应（quantitative polymerase chain reaction，qPCR）检测，用于染色质免疫沉淀（chromatin immunoprecipitation，ChIP）与连续染色质免疫沉淀（sequential chromatin immunoprecipitation，sequential ChIP）分析，以补充图6的相关内容。
用于免疫印迹与染色质免疫沉淀分析的抗体信息可参见补充表S1。
用于克隆与实时定量聚合酶链式反应的引物序列信息可参见补充表S2。
基于DESeq2的差异基因表达分析数据可参见补充表S3。