Cytochrome b sequences isolated from Amphiprion spp.

This dataset consists of 361 cytochrome b sequences isolated from Amphiprion spp. and made available from NCBI GenBank: accession numbers MNB150716 - MN151076.
Abstract [Related Publication]: Hybridization and introgression are evolutionarily significant phenomena breaking down species boundaries. “Hybrid zones” (regions of species overlap and hybridization) enable quantification of hybridization frequency and examination of mechanisms driving and maintaining gene flow. The hybrid anemonefish Amphiprion leucokranos is found where parent species (A. chrysopterus; A. sandaracinos) distributions overlap. Here, we examine geographic variation in hybridization and introgression, and potential impacts on parent species integrity through assessing relative abundance, social group composition, and genetic structure (mtDNA cytochrome b, 21 microsatellite loci) of taxa at three hybrid zone locations: Kimbe Bay (KB) and Kavieng (KA), Papua New Guinea; the Solomon Islands (SO). Relative abundances of and size disparities between parent species apparently drive hybridization frequency, introgression patterns, and genetic composition of taxa. Conspecific groups are most common in KB (65%) where parent species are similarly abundant. Conversely, mixed species groups dominate SO (82%), where A. chrysopterus is more abundant. Hybrids most commonly cohabit with A. sandaracinos in KB (17%), but with A. chrysopterus in KA (22%) and SO (50%). Genetic differentiation (nDNA) analyses indicate that parent species remain distinct, despite ongoing hybridization and hybrids are genetically similar to A. sandaracinos—resulting from persistent backcrossing with this smallest species. This study shows that hybridization outcomes may depend on the social and ecological context in which taxa hybridize, where relative abundance and disparate size of parent species explain the frequency and patterns of hybridization and introgression in the A. leucokranos hybrid zone, reflecting size‐based dominance behaviors of anemonefish social groups.
The full methodology is available in the Open Access publication from the Related Publications link below.

本数据集包含361条从双锯鱼属（Amphiprion spp.）个体中分离得到的细胞色素b序列，数据源自NCBI GenBank数据库，登录号范围为MNB150716至MN151076。

【相关文献摘要】杂交与基因渐渗是打破物种界限的重要进化现象。“杂交带”（物种重叠分布并发生杂交的区域）可用于量化杂交频率，解析驱动并维持基因流动的内在机制。杂交性银背双锯鱼（Amphiprion leucokranos）的自然分布区处于其亲本物种——大堡礁双锯鱼（A. chrysopterus）与萨氏双锯鱼（A. sandaracinos）的分布重叠地带。本研究选取巴布亚新几内亚的金贝湾（KB）、卡维恩（KA）以及所罗门群岛（SO）三处杂交带点位，通过分析类群的相对丰度、社会群体组成以及遗传结构（线粒体DNA（mtDNA）细胞色素b序列、21个微卫星位点），探究杂交与基因渐渗的地理变异特征，及其对亲本物种遗传完整性的潜在影响。

研究结果显示，亲本物种的相对丰度与体型差异似乎主导了杂交频率、基因渐渗模式以及类群的遗传组成。在亲本物种丰度相近的金贝湾，同物种社会群体占比最高（65%）；反之，在大堡礁双锯鱼更为优势的所罗门群岛，混合物种社会群体占比达82%，占据绝对主导地位。在金贝湾，杂交个体多与萨氏双锯鱼共栖（17%）；而在卡维恩（22%）与所罗门群岛（50%），杂交个体则更多与大堡礁双锯鱼共栖。核DNA（nDNA）遗传分化分析表明，尽管存在持续的杂交事件，两个亲本物种仍保持遗传分化；且杂交个体的遗传特征与萨氏双锯鱼更为相似，这一结果源于其与该体型更小的物种持续回交。本研究表明，杂交结局可能取决于类群发生杂交的社会与生态背景：双锯鱼社会群体基于体型的优势等级行为，使得亲本物种的相对丰度与体型差异，可以解释该杂交带中杂交与基因渐渗的频率与模式。

完整的研究方法可通过下方相关文献链接指向的开放获取出版物查阅获取。