Additional file 1 of N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma

Additional file 1: Table S2. Sequences of shRNA&siRNA against specific target in this study. Fig. S1 A Colony formation assay of sunitinib-resistant cell lines and control cell lines with DMSO in 12-well dish for 3 weeks (n = 3). Fig. S2 A TRAF1 pathways in KEGG. B Proteins involed in angiogenesis signaling were mediated by TRAF1 in OS-RC-2 cells. Fig. S3 A and B ChIP assays were used to assess the degree of H3K4me3 within the regions 1-3 of the TRAF1 promoter in 78S and 78R cells.

附加文件1：表S2。本研究中靶向特定靶点的短发夹RNA（shRNA）与小干扰RNA（siRNA）序列。补充图S1A：将舒尼替尼耐药细胞系与对照细胞系置于12孔板中，以二甲基亚砜（DMSO）处理，进行集落形成实验，持续培养3周（n = 3）。补充图S2：A为京都基因与基因组百科全书（KEGG）收录的肿瘤坏死因子受体相关因子1（TRAF1）信号通路；B为OS-RC-2细胞中受TRAF1调控的、参与血管生成信号通路的相关蛋白。补充图S3A与B：采用染色质免疫沉淀（ChIP）实验，检测78S与78R细胞中TRAF1基因启动子1~3号区域内H3K4me3的修饰水平。