Molecular and behavioral consequences of Ube3a gene overdosage in mice

Here, we investigated the neurodevelopmental impact of Ube3a gene overdosage using bacterial artificial chromosome-based transgenic mouse models (Ube3aOE) that recapitulate the increases in Ube3a copy number most often observed in Dup15q.Here, we investigated the neurodevelopmental impact of Ube3a gene overdosage using bacterial artificial chromosome-based transgenic mouse models (Ube3aOE) that recapitulate the increases in Ube3a copy number most often observed in Dup15q. Hereto we performed gene expression profiling analysis using data obtained from RNA-seq of cortical and hippocampal tissue from wildtype and Ube3aOE mice Comparative gene expression profiling analysis of RNA-seq data for cortical and hippocampal brain tissue of 5 wildtype and 5 Ube3aOE mice本研究通过基于细菌人工染色体（bacterial artificial chromosome）构建的转基因小鼠模型（Ube3aOE），探究Ube3a基因剂量过高对神经发育的影响；该模型可精准复刻15q11-q13重复综合征（Dup15q）中最常见的Ube3a拷贝数增加现象。在此基础上，本研究利用野生型与Ube3aOE小鼠皮层及海马组织的RNA-seq数据开展基因表达谱分析，并针对5只野生型小鼠与5只Ube3aOE小鼠的皮层及海马脑组织RNA-seq数据完成对比基因表达谱分析。