Data files describing the systemic screening of 96 Schistosoma mansoni cell surface and secreted antigens produced in a mammalian expression system

In this study, the authors have established an infrastructure for testing a large number of vaccine candidates in mice and used it to screen 96 cell-surface and secreted recombinant proteins from Schistosoma mansoni. This approach, using standardised immunisation and percutaneous infection protocols, allowed comparison of an extensive set of antigens in a systematic manner. This data record consists of nine datasets. Datasets 1. Optimisation of the duration of challenge for percutaneous infection.pzf, 2. Optimisation of the rest period after immunisation.pzf, 4. Half maximal antibody titres.pzf, 5. Correlation Eggs vs Total Adults or Mature Females.pzf, 7. Cohort analysis - Individual data for antigens 10 and 58.pzf and 8. Repeat analysis on larger groups - Proteins 10 and 58.pzf are in .pzf file format. Dataset 3. S. mansoni proteins gels.pptx is in .pptx file format. Dataset 6. Eggs per gram of liver counted across cohorts.xlsx is in .xlsx file format. Dataset RDS-SPRN-00358.xlsx is in .xlsx file format and shows the data contained within the .pzf datasets. Spreadsheet "1. Duration of challenge" shows the data in .pzf file 1. Spreadsheet "2. Rest period" shows the data in .pzf file 2. Spreadsheet "4. Antibody titres" shows the data in .pzf file 4. Spreadsheets "5a. Correlation eggs-adults" and "5b. Correlation eggs-females" show the data in .pzf file 5. Spreadsheets "7a. Cohort-protein 10" and "7b. Cohort-protein 58" show the data in .pzf file 7. Spreadsheets "8a. Repeat-protein 10" and "8b. Repeat-protein 58" show the data in .pzf file 8. Dataset 1. Optimisation of the duration of challenge for percutaneous infection.pzf includes data on the optimisation of the conditions of percutaneous challenge in BALB/C female mice by testing different durations of exposure to the parasite. Dataset 2. Optimisation of the rest period after immunisation.pzf includes data on the optimisation of the conditions of percutaneous challenge in BALB/C female mice by testing different rest periods between immunisations and infection challenge. Dataset 3. S. mansoni proteins gels.pptx includes SDS-page data on 96 recombinant S. mansoni antigens that were produced in mammalian cells and purified by Ni2+-affinity purification (their integrity was checked by SDS-page before immunisations in BALB/C female mice). Dataset 4. Half maximal antibody titres.pzf includes data on the half-maximal antibody titres. These were derived by immunising each group of mice (5 animals in each group) with an antigen of interest and one control who received PBS. Eight days after the final immunisations, blood samples were collected from each animal and the half-maximal antibody titres against the antigen of interest were determined. Dataset 5. Correlation Eggs vs Total Adults or Mature Females.pzf includes data on the number of eggs and the number of mature female worms counted on euthanized, immunised animals that were challenged by percutaneous infection with S. mansoni cercariae. Dataset 6. Eggs per gram of liver counted across cohorts.xlsx includes data on the number of eggs per gram of liver, that were counted across all cohorts of animals. Dataset 7. Cohort analysis - Individual data for antigens 10 and 58.pzf includes data on individual data points in cohorts corresponding to antigens 10 and 58, for which a statistically significant difference in the number of eggs per gram of liver was observed by ANOVA analysis compared to the other groups from the same cohort. Dataset 8. Repeat analysis on larger groups - Proteins 10 and 58.pzf includes data from repeat experiments when immunisations and challenges were repeated with antigens 10 and 58 on a larger set of animals comprising of twelve experimental and six control animals. Study aims and methodology: The study aimed to test the protective efficacy of 96 secreted antigen proteins from Schistosoma mansoni, in immunised mice, against infection with S. mansoni. Recombinant antigens were produced by transient transfection of human embryonic kidney (HEK)-293-E or 6E cells. All animal experiments were performed in accordance with UK Home Office regulations under project licenses P77E8A062 and PD3DA8D1F. For each purified antigen, a group of 5 female BALB/C mice was immunised intraperitoneally. In each group, a sixth animal was used as a cage control. After a 4-week rest period, animals were challenged with S. mansoni cercariae. Forty-two days after the challenge, mice were euthanized and S. mansoni worms were collected from individual mice. Liver tissues were weighed and the number of eggs per gram of liver was calculated. Following immunisation, blood samples were collected from each animal and antibody titres were determined using enzyme-linked immunosorbent assay (ELISA). For more details on the methodology and statistical analysis, see the published article. Software needed to access datasets: Datasets in the. pzf file format require the GraphPad Prism software to be accessed.

本研究中，研究者构建了一套可在小鼠体内大规模筛选疫苗候选株的实验体系，并利用该体系对曼氏血吸虫（Schistosoma mansoni）的96种细胞表面及分泌型重组蛋白进行了筛选。本研究采用标准化免疫方案与经皮感染攻毒规程，可系统性对比分析大量抗原的免疫保护效果。本数据集共包含9个子数据集：
1. 《经皮感染攻毒时长优化.pzf》（Optimisation of the duration of challenge for percutaneous infection.pzf）
2. 《免疫后休整周期优化.pzf》（Optimisation of the rest period after immunisation.pzf）
3. 《曼氏血吸虫蛋白凝胶图.pptx》（S. mansoni proteins gels.pptx）
4. 《半数抗体效价.pzf》（Half maximal antibody titres.pzf）
5. 《虫卵数与成虫总数/成熟雌虫数的相关性.pzf》（Correlation Eggs vs Total Adults or Mature Females.pzf）
6. 《各组肝脏虫卵计数.xlsx》（Eggs per gram of liver counted across cohorts.xlsx）
7. 《队列分析——抗原10与58的个体数据.pzf》（Cohort analysis - Individual data for antigens 10 and 58.pzf）
8. 《大样本重复分析——蛋白10与58.pzf》（Repeat analysis on larger groups - Proteins 10 and 58.pzf）
此外，数据集RDS-SPRN-00358.xlsx同样为.xlsx格式，包含各.pzf数据集对应的原始数据：其工作表"1. 攻毒时长"对应.pzf文件1的数据，"2. 休整周期"对应.pzf文件2的数据，"4. 抗体效价"对应.pzf文件4的数据；工作表"5a. 虫卵-成虫相关性"与"5b. 虫卵-雌虫相关性"对应.pzf文件5的数据；工作表"7a. 队列-蛋白10"与"7b. 队列-蛋白58"对应.pzf文件7的数据；工作表"8a. 重复实验-蛋白10"与"8b. 重复实验-蛋白58"对应.pzf文件8的数据。

各子数据集详情如下：
《经皮感染攻毒时长优化.pzf》包含BALB/C雌性小鼠经皮感染曼氏血吸虫时，通过调整不同寄生虫暴露时长优化攻毒条件的相关数据。
《免疫后休整周期优化.pzf》包含通过调整免疫与攻毒间的不同休整周期，优化BALB/C雌性小鼠经皮感染攻毒条件的相关数据。
《曼氏血吸虫蛋白凝胶图.pptx》包含96种重组曼氏血吸虫抗原的SDS-聚丙烯酰胺凝胶电泳（SDS-page）数据，这些抗原通过哺乳动物细胞表达、Ni²⁺亲和层析纯化，并在免疫BALB/C雌性小鼠前通过SDS-page验证了蛋白完整性。
《半数抗体效价.pzf》包含半数最大抗体效价相关数据：将每组5只小鼠分别免疫目标抗原，另设一组注射磷酸盐缓冲液（PBS）作为对照；末次免疫后8天采集每只小鼠的血液样本，测定其针对目标抗原的半数最大抗体效价。
《虫卵数与成虫总数/成熟雌虫数的相关性.pzf》包含经皮感染曼氏血吸虫尾蚴的免疫小鼠处死后，其体内虫卵数与成虫总数、成熟雌虫数的计数数据。
《各组肝脏虫卵计数.xlsx》包含所有受试队列小鼠的肝脏组织每克虫卵数的计数数据。
《队列分析——抗原10与58的个体数据.pzf》包含抗原10与58对应队列的个体数据点；经方差分析（ANOVA）证实，该两组的肝脏每克虫卵数与同队列其余组别存在统计学差异。
《大样本重复分析——蛋白10与58.pzf》包含重复实验的数据：使用抗原10与58对更大样本量的小鼠进行免疫与攻毒，其中实验组12只、对照组6只。

研究目标与方法：本研究旨在验证96种曼氏血吸虫分泌型抗原蛋白在免疫小鼠体内的抗曼氏血吸虫感染保护效力。重组抗原通过瞬时转染人胚肾（HEK）-293-E或6E细胞制备。所有动物实验均遵循英国内政部相关规定，在项目许可P77E8A062与PD3DA8D1F框架下开展。对于每一种纯化抗原，将5只雌性BALB/C小鼠经腹腔免疫，另设1只同笼小鼠作为对照。免疫4周休整期后，对小鼠攻以曼氏血吸虫尾蚴。攻毒42天后处死小鼠，收集每只小鼠体内的曼氏血吸虫成虫；称量肝脏组织并计算每克肝脏的虫卵数。免疫后采集每只小鼠的血液样本，通过酶联免疫吸附试验（ELISA）测定抗体效价。如需了解更多方法学与统计学分析细节，请参阅已发表的相关论文。

数据集访问所需软件：.pzf格式的数据集需使用GraphPad Prism软件打开。