Phormidium pseudopriestleyi laboratory culture

In Lake Fryxell, Antarctica, the benthic, filamentous cyanobacterium Phormidium pseudopriestleyi creates a 1-2 mm thick layer of 50 umol L-1 O2 in otherwise sulfidic water, demonstrating that is sustains oxygenic photosynthesis in the presence of sulfide. A sample of P. pseudopriestleyi was taken from 9.8 m depth in Lake Fryxell. The culture was stored at ambient indoor light at the lakeside laboratory for approximately ten days and then shipped to the Natural History Museum, where it was grown in BG11 liquid medium at 10C, and 24 h light. DNA was extracted from the enrichment culture using the MoBio Powerbio DNA extraction kit according to the manufacturer's instructions. The culture was sequenced on the Illumina HiSeq platform at the University of Michigan DNA Sequencing Core.