LINE1 and PRC2 control nucleolar organization and repression of the 8C-state in human ESCs [RNA-Seq_Primed_H9_L1KD]

To study L1 knockdown effect in mTeSR primed hES cells Overall design: In this study, we investigated LINE1 (L1), a major family of transposon elements (TEs) in the human genome, in primed hES cells for its roles in regulating 8-cell (8C) stage-specific totipotent genes. mTeSR cultured primed H9 hES cells were transfected with 6-FAM (Fluorescein) labeled non-specific targeting ASO (ASO-Ctr), L1 targeting sense oligo (SO-L1) and anti-sense oligo (ASO-L1), respectively. Cells were maintained in the RSeT media post transfection. 48 hours later, FAM+ cells were FACS collected and immediately processed for RNA extraction and RNA-seq library prep.