The role of curcumin during pregnancy on the exposed fetuses’ tissues of Wistar rats to electromagnetic field

To investigate curcumin (CUR) as the protector against the harmful effects of low-frequency electromagnetic field(LF- EMF, 50 Hz) during pregnancy period, 5 males and 15 females of Wistar rat mated and vaginal plaques were observed. Then, the pregnant rats were divided into six groups. During pregnancy(21 days), the EMF group was exposed to EMF for 30 min/day, the CUR group received a single dose of 50 mg/kg/daily CUR intraperitoneal, the EMF+CUR group was injected CUR and exposed to EMF daily. The DMSO(dimethyl sulfoxide) group was injected solvent of CUR (DMSO) intraperitoneal with the same volume of CUR solvent, the sham group was placed through the solenoid in the same conditions as the first group without exposure and the control group was kept in their cage in normal condition. After four weeks, babies born were divided according to the mother groups and sacrificed. Then, the three tissues injuries were investigated. EMF exposure led to an increase in outstanding necrotic areas in hippocampal tissue, an increase in the amount of hyperemia(<i>p</i> = 0.017) and necrotic(<i>p</i> = 0.005) in kidneys, and degeneration in liver tissue(<i>p</i> = 0.007) in the EMF group compared with EMF+CUR groups. A single dose of CUR daily during pregnancy can protect these tissues from injuries caused by LF-EMF exposure in rat fetuses. Electromagnetic fields (EMFs) are able to penetrate and be absorbed by the body. The researchers showed that these radiations might be harmful and lead to cancers, cardiovascular diseases, mental disorders, and fetal abnormalities. Curcumin as an active component in turmeric has anti-inflammatory, antioxidant and anti-hyperlipidemia properties. It can protect the body against diseases such as arthritis, anxiety, and metabolic syndrome. This study examined the effects of curcumin as the protector against the harmful effects of EMF (50Hz) during pregnancy period. So the pregnant rats were divided into six groups. During pregnancy, a group was exposed to EMF for 30 min/day, the second group was injected a dose of curcumin 50mg/kg/daily, the third group was injected curcumin and exposed to EMF daily. The fourth group was injected a curcumin solvent dose, the sham group was placed through the field generator in the same conditions as the first group without exposure and the control group was kept in their cage in normal condition. After four weeks, babies born were divided according to the mother groups and sacrificed. Then, the liver, kidney, and hippocampal tissues were investigated. EMF exposure led to an outstanding increase in necrotic areas in hippocampal tissue, a notable increase in the amount of hyperemia and necrosis in kidneys, and degeneration in liver tissue(<i>p</i>=0.007) in the EMF group compared with the third group that was exposed to EMF and received curcumin. A single dose of curcumin daily during pregnancy can protect these tissues from injuries caused by EMF(50Hz) exposure in rat fetuses.

为探究姜黄素（curcumin, CUR）对妊娠期低频电磁场（low-frequency electromagnetic field, LF-EMF, 50 Hz）所致损伤的保护作用，本实验将5只雄性与15只雌性Wistar大鼠合笼饲养，观察到阴道栓后确认妊娠。随后将孕鼠分为6组。在为期21天的妊娠期内，电磁场暴露组每日接受30分钟电磁场暴露；姜黄素组每日单次腹腔注射50 mg/kg姜黄素；电磁场+姜黄素组每日同时接受姜黄素注射与电磁场暴露；二甲基亚砜（dimethyl sulfoxide, DMSO）组以与姜黄素溶剂等体积的溶剂进行腹腔注射；假照射组置于与电磁场暴露组相同的电磁线圈环境中，但不接受暴露；对照组则正常饲养于笼中。
分娩四周后，将产下的仔鼠按母鼠分组并实施安乐死，随后检测三种组织的损伤情况。与电磁场+姜黄素组相比，电磁场暴露组仔鼠的海马组织坏死面积显著升高，肾脏组织充血量（*p*=0.017）与坏死灶数量（*p*=0.005）均显著增加，肝脏组织出现变性（*p*=0.007）。妊娠期每日单次给予姜黄素，可保护仔鼠的上述三种组织免受50 Hz低频电磁场暴露所致的损伤。
电磁场（EMFs）可穿透机体并被其吸收。已有研究表明，此类辐射可能具有危害，可诱发癌症、心血管疾病、精神障碍及胎儿畸形。姜黄素作为姜黄中的活性成分，具有抗炎、抗氧化及抗高脂血症的药理活性，可用于保护机体抵御关节炎、焦虑症及代谢综合征等疾病。
本实验探究了姜黄素对妊娠期50 Hz电磁场所致损伤的保护作用。将孕鼠分为6组：在21天妊娠期内，第一组每日接受30分钟电磁场暴露；第二组每日单次腹腔注射50 mg/kg姜黄素；第三组每日同时接受姜黄素注射与电磁场暴露；第四组注射与姜黄素溶剂等体积的溶剂；假照射组置于与第一组相同的场发生器环境中，但不接受暴露；对照组正常饲养于笼中。分娩四周后，将仔鼠按母鼠分组并实施安乐死，随后检测肝脏、肾脏及海马组织的损伤情况。与第三组（电磁场暴露+姜黄素注射组）相比，电磁场暴露组仔鼠的海马组织坏死面积显著增加，肾脏组织充血量与坏死灶数量显著升高，肝脏组织出现变性（*p*=0.007）。妊娠期每日单次给予姜黄素，可保护仔鼠的上述三种组织免受50 Hz电磁场暴露所致的损伤。